DL-1,4-Dithiothreitol; Dithiothreitol; DTT

Product Details

CAS No:3483-12-3

MF:C4H10O2S2

Appearance:white or off-white crystalline powder

Specification:98.5%

UN number:-

Package:1/5/10/25kgs/drum

Supply Capacity:MT

Synonyms:DL-1,4-Dithiothreitol, 1M solution in water, for molecular biology;Dithiothreitol ,98%;DL-1,4-Dithiothreitol,DTT;DL-Dithiothreitol ,99% [for Molecular biology];DL-1,4-Dithiothreitol, DNAse, RNAse and Protease free, for Molecular b;DL-1,4-Dithiothreitol, DNAse, RNAse and Protease free, for Molecular biology, 99+% 1GR;DL-1,4-Dithiothreitol, for biocheMistry, 99% 1GR;DL-1,4-Dithiothreitol, for biocheMistry, 99% 5GR; Dithiothreitol; 1,4-Dithiothreitol; 3483-12-3; Cleland reagent; Sputolysin; Cleland's reagent; (2R,3R)-1,4-disulfanylbutane-2,3-diol; Threitol, 1,4-dithio-; T8ID5YZU6Y; 2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel-; Clelands reagent; Dithiotreitol; 2,3-Butanediol, 1,4-dimercapto-, DL-threo-; threo-1,4-Dimercapto-2,3-butanediol; WR-34678; (R*,R*)-(1)-1,4-Dimercaptobutane-2,3-diol; (R*,R*)-(+-)-1,4-Dimercapto-2,3-butanediol; 2,3-BUTANEDIOL, 1,4-DIMERCAPTO-, D-threo-; DTXSID5041017; Reagent, Cleland; D-threo-1,4-Dimercapto-2,3-butanediol; WR 34678; Reagent, Cleland's; CHEBI:18320; RefChem:416957; GlyTouCan:G47410OV; DTXCID00820548; G47410OV; 2,3-Butanediol, 1,4-dimercapto-, (R*,R*)-(+-)-; 222-468-7; 248-531-9; L-Dithiothreitol; 16096-97-2; L-1,4-Dithiothreitol; L-Dtt; 2,3-DIHYDROXY-1,4-DITHIOBUTANE; dl-Dithiothreitol; (2R,3R)-1,4-bis(sulfanyl)butane-2,3-diol; (2R,3R)-1,4-dimercaptobutane-2,3-diol; MFCD00064305; 2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-; L-threo-1,4-Dimercapto-2,3-butanediol; DL-threo-1,4-Dimercapto-2,3-butanediol; 1,4-Dithio-dl-threitol; DTT; Threitol, 1,4-dithio-, DL-; rac-Dithiothreitol; L-Cleland's Reagent; Mucolyse; LDtt; Threitol, dithio-; (2R,3R)-rel-1,4-Dimercapto-2,3-butanediol; UNII-T8ID5YZU6Y; CLELAND REAGENT RACEMIC; SCHEMBL538995; CCRIS 3617; CHEMBL406270; orb1218706; orb1306721; CHEBI:42106; HSDB 8422; VHJLVAABSRFDPM-IMJSIDKUSA-N; 1,4-DITHIOTHREITOL [MI]; DTXSID501316708; L-(-)-Dithiothreitol, >=95%; L-1,4-disulfanylbutane-2,3-diol; EINECS 222-468-7; EINECS 248-531-9; AKOS028109573; DB04447; FD02370; BRN 1719757; AS-64942; HY-15917; SY234556; (R*,R*)-1,4-Dimercaptobutane-2,3-diol; AI3-62064; D1589; NS00068394; 1,4-DIMERCAPTO-2,3-BUTANEDIOL, DL-; C00265; D70251; T20224; (2R,3R)-(-)-1,4-Dimercapto-2,3-butanediol; (2R,3R)-(-)-2,3-Dihydroxy-1,4-butanedithiol; 3-01-00-02360 (Beilstein Handbook Reference); F493710; Q27104490; 2,3-Butanediol, 1,4-dimercapto-, (R*,R*)- (+-)-; 2,3-Butanediol, 1,4-dimercapto-, (theta,theta)-(+/-)-; 2,3-BUTANEDIOL, 1,4-DIMERCAPTO-, (R*,R*)- (+/-)-; L-1,4-Dithiothreitol; (2R,3R)-1,4-Dimercapto-2,3-butanediol

Product application:DL-1,4-Dithiothreitol,3483-12-3,DTT,Cleland's reagent,threo-1,4-Dimercapto-2,3-butanediol,Dithiothreitol,Molecular biology grade DTT,Electrophoresis grade DTT,Protein reducing agent,Disulfide bond reducing agent,Sulfhydryl protecting agent,Protein refolding reagent,ADC conjugation reducing agent,Antibody disulfide cleavage agent,Enzyme stabilizer,Reverse transcriptase protector,In vitro diagnostic IVD reagent,Low oxidized DTT,Ultra pure DTT 99.5%,DTT 1M solution,CAS 3483-12-3 supplier,DL-1,4-Dithiothreitol manufacturer China,Suzhou Health Chemicals biochemical reagents,Dithiothreitol wholesale bulk export

 

DL-1,4-Dithiothreitol finds widespread and practical utility across biomedical research, proteomic structural analysis, industrial recombinant protein refolding campaigns, targeted antibody-drug conjugate bioconjugation workflows, nucleic acid amplification diagnostic manufacturing, and functional biopolymer synthesis sectors. In protein chemistry and analytical proteomics, universally designated as DTT or Cleland's reagent, it functions as the standard stoichiometric sulfhydryl protecting agent and disulfide bond reducer; its molecular architecture incorporates two terminal thiol groups and two central hydroxyl groups that undergo conformational cyclization upon oxidation to form a thermodynamically favored six-membered cyclic disulfide ring; this energetic intramolecular driving force establishes an oxidation-reduction potential that drives the quantitative cleavage of intermolecular and intramolecular covalent cystine disulfides at neutral pH; it is formulated into SDS-PAGE denaturation sample buffers and two-dimensional electrophoresis gels to fully linearize complex polypeptide chains, ensuring reliable molecular weight resolution and reproducible mass spectrometry identification; in the clinical biomanufacturing of antibody-drug conjugates, it is utilized for the site-specific partial reduction of native interchain disulfide bridges within monoclonal antibody hinge domains, liberating reactive free cysteine thiols that enable subsequent alkylation with cytotoxic drug-linker constructs to achieve uniform drug-to-antibody ratios; in commercial bioprocessing utilizing bacterial expression hosts, it serves as a critical component in denaturation-refolding buffers for processing insoluble inclusion bodies of recombinant insulin, human cytokines, and industrial biocatalysts, suppressing non-native disulfide shuffling to promote correct native conformation recovery; in molecular diagnostics and genomic enzymology, it acts as a functional stabilizer in reaction buffers for reverse transcriptases, RNA polymerases, and DNA polymerases by preserving catalytic-cleft cysteine thiols in their active reduced state, shielding critical enzymes from trace dissolved oxygen and heavy-metal oxidation to improve detection sensitivity and amplification linearity in clinical real-time PCR kits and next-generation sequencing library preparation protocols; in sample preparation and nucleic acid extraction workflows, its rapid reduction kinetics accelerate the complete inactivation of endogenous ribonucleases and proteases in raw biological specimens, protecting intact high-molecular-weight viral and eukaryotic RNA from degradation; furthermore, in cosmetics cold-wave hair waving lotions, microfluidic biosensor surface activation, and thiol-reactive self-assembled nanomaterials, DL-1,4-Dithiothreitol operates as an essential biochemical reagent delivering reliable reducing efficiency and reproducible processing performance across industries.